e-RGA: enhanced Reference Guided Assembly of Complex Genomes

Francesco Vezzi, Federica Cattonaro, Alberto Policriti

Abstract


Next Generation Sequencing has totally changed genomics: we are able to produce huge amounts of data at an incredibly low cost compared to Sanger sequencing. Despite this, some old problems have become even more difficult, de novo assembly being on top of this list. Despite efforts to design tools able to assemble, de novo, an organism sequenced with short reads, the results are still far from those achievable with long reads. In this paper, we propose a novel method that aims to improve de novo assembly in the presence of a closely related reference. The idea is to combine de novo and reference-guided assembly in order to obtain enhanced results.

Keywords


de novo assembly; reference guided assembly; next generation sequencing

Full Text:

HTML PDF


DOI: http://dx.doi.org/10.14806/ej.17.1.208

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.