AlienTrimmer v3 quickly and accurately filters out troublesome bases from nanopore sequencing read ends

Authors

  • Alexis Criscuolo Institut Pasteur, Université Paris Cité, GIPhy – Genome Informatics & Phylogenetics, Biological Resource Centre of Institut Pasteur, Paris https://orcid.org/0000-0002-8212-5215

DOI:

https://doi.org/10.14806/ej.31.0.1078

Keywords:

trimming, nanopore, sequencing

Abstract

Since its first release in 2013, the sequence read trimming tool AlienTrimmer has been continuously improved, for use in various practical cases. In this context, the growing popularity of nanopore sequencing has made it necessary to update AlienTrimmer to deal with such long reads. New features were then implemented while guaranteeing that AlienTrimmer can still run fast. As illustrated in this note, the last release of AlienTrimmer can now filter out troublesome bases (i.e., low-quality and/or exogenous bases) from nanopore read ends with both first-rate accuracy and speed.

 

Availability: the source code of AlienTrimmer is available at https://gitlab.pasteur.fr/GIPhy/AlienTrimmer

 

 

 

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Published

2026-05-07

Issue

Section

Technical Notes