Comparison of oligonucleotide microarray and RNA-seq technologies in the context of gene expression analysis

Authors

  • Nicolas Sierro Philip Morris R&D, Neuchatel
  • Florian Martin Philip Morris R&D, Neuchatel
  • Carine Poussin Philip Morris R&D, Neuchatel
  • Julia Hoeng Philip Morris R&D, Neuchatel
  • Nikolai V. Ivanov Philip Morris R&D, Neuchatel

DOI:

https://doi.org/10.14806/ej.19.A.642

Keywords:

RNA-seq, DNA microarray, gene expression analysis

Abstract

For more than a decade, the microarray technology has been widely used for genome-wide gene expression profiling of several experimental systems. Transcript abundance measurement is currently revolutionized by RNA-seq technology which enables the sequencing of the whole transcriptome. The purpose of the described research was to compare the Affymetrix GeneTitan array and the Illumina HiSeq-2000 sequencing technologies in the context of gene expression analysis. Overall, this study shows that RNA-seq is a very powerful technology since it is more sensitive to detect low and differential expressed transcripts/genes compared to the Affymetrix GeneTitan technology.

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Published

2013-04-08

Issue

Vol. 19: Supplement A

Section

Posters

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